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Cell viability Cell growth was assessed using the trypan blue exclusion assay

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India: The country, which has the second-largest number of traditional tobacco smokers after China, has not yet established specific regulations for nicotine pouches but has enforced strict controls on vaping products

The bystander asked the agents, "Can I check a pulse?" An agent replied, "No, back up now!" The bystander said, "I'm a physician!" An agent replied, "I don't care." Another agent said EMS was on the way, and another said they had their own medics on scene

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However, their strong potency and chemical diversity can also lead to serious negative effects, such as