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However, the effect of acetaldehyde seems not to be mediated by opioid receptors in the CNS and the substance does not cause physiological addictiveness (Charles et al

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In each case, the microsomal suspension (1 mg protein) was incubated with 50 l smoke solution in a final volume of 20 l of 50 mM potassium phosphate buffer, pH 7.4 for 2 hours at 37 C