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At the end of this incubation period, the cultures were centrifuged 10 min at 13,000 rpm, the supernatants were discarded, and each pellet was suspended in 0.6 ml of lysis buffer (1 M glycerol, 0.1 M EDTA)

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Supplementary material The Supplementary Material for this article can be found online at: Supplementary Figure S1 Mean Serum Cotinine Concentration by Time Period

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The camera crew packed up before Kelly could even reach the stage

doi: 10.3390/ijerph14101095 113