Using bacteriostatic water , rotating injection sites, and ensuring sterile technique all reduce the likelihood and severity of local reactions
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This procedure involved removing the supernatant and resuspending the mitochondrial pellet in an isolation buffer of 250 mM sucrose, 0.5 mM EDTA, and 10 mM Tris-HCl to achieve a final protein concentration of 20-25 mg/ml [2]
54 EMA (2025)