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However, this benefit must be balanced with the risk of younger adolescents, who are not current smokers and who were unlikely to try combustible cigarettes, experimenting with e-cigarettes and developing nicotine addictions

Methods in enzymology 233 , 357363 (1994)

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After washing twice in 1 PBS, cells were resuspended in ALI expansion medium with RI and seeded onto bovine collagen-coated 6.5 mm transwell inserts (2 10 4 cells/insert) containing ALI Expansion medium with RI in the basolateral chamber (day 5)