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Bacteria were washed off the agar with 2 mL M9 buffer, subjected to OD 600 measurement using a DS-11 FX+ spectrophotometer (DeNovix), and bacterial density determined by serial dilution, plating on agar plates, and counting colony-forming units (CFU) 64

After the microspheres are washed in a quench liquid, they are isolated, as through a sieve, and dried

Transfer the supernatant to a new tube and add ddH 2 O to reduce the SSA concentration to 0.5 % for the assay

Ben Lynch: Two were slow or--

4HNE released from ferroptotic cells induces stress response pathways and Akt signaling in hepatocytes, resulting in lipid droplet accumulation and insulin resistance [42]

Two weeks is too early to expect significant weight loss in most cases