8 By binding to and eliminated ROS, glutathione can diminish oxidative stress
At baseline, GPx1 deficient cardiomyocyte and liver mitochondria show increased mitochondrial ROS production [41, 174], with measurable alterations in respiration in liver mitochondria
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BAL fluid, cell lysates, or apical culture supernatants were incubated with glutathione peroxidase (Sigma-Aldrich, G6137), cumene hydroperoxide (Sigma-Aldrich, 247502), glutathione reductase (Sigma-Aldrich, G9297), and NADPH (Sigma-Aldrich, N1630) for 30 minutes at 25C