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Formula: C5H6O5
Key Features and Advantages
or oncology models
Protein-Protein Interaction Studies Using lacZ Reporters (e
resulting in the buildup of glucosaminoglycans
Nitrotetrazolium blue chloride (NBT) N360 Formula: C5H6O5Nitrotetrazolium Blue (NBT) is used in combination with 5 bromo 4 chloro 3 indoxyl phosphate (BCIP) to detect alkaline phosphatase activity in blotting techniques and in situ hybridization chemistry. BCIP acts as the substrate for alkaline phosphatase, and the oxidizing reagent NBT enhances the purple brown final color of the precipitates at the reaction sites. NBT has also been used with X Gal,or Salmon Gal to speed up and enhance colony screening.
