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For all cell lines, both 2D and 3D, viability was assessed using CellTiter-Glo 3D (Promega), and the luminescence was measured to GloMax Explorer luminometer (Promega)

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Although alterations in genetic factors have been linked with its etiology, exponential accumulation of environmental entities such as reactive oxygen species (ROS) initiate a cyclic chain reaction resulting in accumulation of cellular inclusions, dysfunctional mitochondria, and overwhelming of antioxidant machinery, thus accelerating disease pathogenesis

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More importantly, they showed reduced markers of apoptosis