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Notably, in their HEK293 cell line, Ser363 was constitutively phosphorylated but showed no increase with opioid agonists, and Thr370 could be phosphorylated by stimulation of PKC activity (see below) in addition to DAMGO

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At the same time, the elevation of p-CREB induced by BHBA was also decreased in KN-62-treated neurons, suggesting that the BHBA-induced rise of p-CREB levels depends on the activation of Ca 2+ /CaM/CaMKII (Figure 4B)

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