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Moreover, nuclear IGF-IR promotes slug expression through phosphorylating histone H3 at Y41 [90].Thus, nuclear IGF-IR has both kinase-dependent and kinase-independent functions

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Typically no

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Thereafter, the supernatant was transferred to a pre-chilled microfuge tube, left on ice in darkness for 15 min, and then passed through a 0.45 m PTFE filter