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Computational investigation of cholesterol binding sites on mitochondrial VDAC

06h40min Hrs

After centrifugation (1600 g, 4 C for 5 min), supernatant was diluted by two volumes of NET-2 buffer (50 mM Tris-HCl (pH=7.5), 150 mM NaCl, 0.05% NP-40, 1x protease and 1x phosphatase inhibitors) and pre-cleared by protein G Dynabeads at 4 C for 1 h

if a pill is taken in the morning on the 1st day, try to follow the same sequence the following days).

It has been studied extensively and is considered one of the most important antioxidants in the human body

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