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Cell viability and proliferation assays The transfected K-562 cells with or without cell sorting and the negative control cells transfected with empty plasmids pcDNA3.1-GST DAF or pcDNA3.1-GST CEAM7 were counted and seeded into 96-well plates in triplicates at a density of 1,000 per well

He, J., Wasa, M., Chan, K

Chaves, C., Bruinstroop, E., Refetoff, S., Yen, P

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Have certain medical conditions: Problems that can restrict vitamin B12 absorption include pernicious anemia (a rare autoimmune disease that keeps the body from making a protein necessary to absorb B12), digestive illnesses, such as celiac and Crohns diseases, and immune disorders such as lupus, among others