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Direct investigations under hypothyroid conditions are limited, with only a few studies using EAT models or TR-deficient mice suggesting a potential link between hypothyroidism and ECM alterations

Wandel, C., Kim, R

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Mice that received NAC in the drinking water (10 mg/ml) starting at 48 hr after methylmercury administration excreted from 47 to 54% of the 203Hg in urine over the subsequent 48 hr, as compared to 4-10% excretion in control animals

Cell transfection When HUVECs confluency met approximately 6070%, cells in 6-well plates were transfected with plasmid DNA or siRNA for overexpression or knockdown of GSDMD using Lipofectamine TM 3000 (Invitrogen)