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Under physiological conditions, the Neh2 structural domain of Nrf2 binds to Kelch-like ECH-associated protein 1 (Keap1) in the cytoplasm, facilitating Nrf2 ubiquitination and subsequent degradation ( 3.1.3 TRPM4 channels and endothelial dysfunction in atherosclerosis Ye et al

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NMR sample preparation The extract was dissolved in 700 l of deuterated phosphate buffer (25 mM Na 2 DPO 4 , pH 7.0 in D 2 O) with 0.2 mM DSS as internal standard