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The samples were alkylated for 45 min at room temperature, mixed with 15 L non-reducing Laemmli buffer (4% SDS, 120 mM Tris/HCl p H 6.8, 20% glycerol, 0.02% bromophenol blue) and subjected to 12% SDS PAGE

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erefore, restoration of gene pattern characteristic of healthy stem cells, which leads to activation of integrins and p cellular pathways may be another target of GHKs gene modulatory activity relevant to skin regeneration [,]