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Alteration of tight junction gene expression by calcium- and vitamin D-deficient diet in the duodenum of calbindin-null mice

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After equilibration, the straws were placed in a programmable freezer (Freeze Control, Cryologic, Australia) pre-cooled to 5 C, the temperature was lowered from room temperature to 80 C at a rate of 12 C per min, after which the straws were plunged into liquid nitrogen for long-term storage

MNT spoke with Hsinlin Thomas Cheng, MD, PhD, senior neurologist in the Department of Neurology at Massachusetts General Hospital and assistant professor at Harvard Medical School, about this study