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Intracellular ROS production Intracellular reactive oxygen species (ROS) levels were measured in treated cells using the oxidant-sensitive probe 2,7dichlorofluorescein diacetate [H 2 DCFDA] 11

Proteins produced in vitro were subjected to interaction assays for 2 hours at 4C under mild rotation with Drosophila nuclear extracts or recombinant protein produced and purified in vitro

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Finally, KOs associated with propanoate metabolism, protein export and bacterial secretion system, thiamine metabolism, glutathione metabolism, and fatty acid biosynthesis, together with cationic antimicrobial peptide and vancomycin resistance were significantly depleted in chickens with improved NR

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