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& Hanigan, M

The samples were stained with hematoxylin-eosin according to automated Sakura Tissue-Tek DRS 2000 Slide Stainer protocol ( accessed on 6 June 2021) following procedural steps: rehydration to distilled water

Natural compounds such as piperlongumine and cotylenin A exhibit synergistic therapeutic efficacy with SAS, which suggest that the triple combined treatment with piperlongumine, piperlongumine and SAS is highly effective against pancreatic cancer (Yamaguchi et al., 2018)

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The animals were then perfused with cold saline to free them of residual blood, after which liver and other tissues were collected immediately, weighed, quickly diced, and frozen in liquid nitrogen at 80 C

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