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aureus using doses of 4347 J cm at high concentrations of 300750 g/mL for GO and 446.3595 g/mL for nGO

To deplete gut microbiota, animals received a cocktail of 100 g/mL neomycin, 50 g/mL streptomycin, 100 g/mL ampicillin, 50 g/mL vancomycin, 100 g/mL metronidazole, 1 mg/mL bacitracin, 125 g/mL ciprofloxacin, and 100 g/mL ceftazidime (Sigma-Aldrich) in the drinking water (69)

For instance, chronic intermittent ethanol (CIE) exposurea widely used model mimicking binge-drinking patternsinduces persistent anhedonia and despair-like behaviors in mice, as evidenced by reduced sucrose preference in the two-bottle choice test and increased immobility in the forced swim test (FST)

We emphasize that proper medical supervision is critical, and any medication should be sourced from verified, regulated channels to ensure quality, safety, and effectiveness

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