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Int J Mol Med 31(1):265271 Del Campo-Rota IM, Delgado-Casillas OM, Ibarra A (2024) Cognitive impairment induced by gestational diabetes: implications of oxidative stress as an inducing mechanism

J Environ Sci Health B

What none of them has, alone or blended, is a completed human trial, so the blend belongs firmly in the research-and-hypothesis column rather than the proven-protocol one

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sgRNA (CAGCCAAAATCACAGAATGA) targeting N-terminus of SNIP1 gene was cloned into pX330-BbsI-PITCh (addgene #127875) 121 and microhomology arms surrounding CRISPR-Cas9 cleavage site (each 20 bp long) were introduced into donor plasmid pCRIS-PITChv2-Puro-dTAG (addgene #91793) 71

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