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The antibodies specific for Np63 (619502, Biolegend, 1:50) or normal rabbit IgG (#2927, CST, 1:50) were used, as described previously [40]

At 48 h, apoptosis was evaluated with Annexin V (1:500 v/v, Invitrogen) and DAPI (0.1 g/mL, Sigma-Aldrich) staining

The effects appear linked to neutrophil recruitment regulation

[2] [3] This approach requires careful consideration of your overall treatment goals, other medical conditions, and NICE guidance for your specific situation

The differences are in manufacturing QC, stopper quality, and labeling consistency