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Fifty or more percent of patients treated with immune checkpoint inhibitors experience some side effects, from rash and local inflammation that can be treated with steroids and/or by temporarily discontinuing the treatment, to severe side effects, such as type 1 diabetes (T1D), thyroid and cardiac issues

Maccani A, Landes N, Stadlmayr G, Maresch D, Leitner C, Maurer M, Gasser B, Ernst W, Kunert R, Mattanovich D (2014) Pichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher spacetime yields for less complex proteins

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Briefly, sitting drop crystallisations were set up in 96-well trays using a Cartesian robot with a reservoir solution of 1.5 M ammonium sulfate and 0.1 M Tris at pH 8.5, each crystallisation drop containing 100 nL each of protein and reservoir solutions

J Neurochem 2000

A 36-fold loss in potency from changing a single stereocenter