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then, 400 l of extraction solution (methanol:acetonitrile = 1:1) was added before vortexing and mixing for 30 s, followed by ultrasonication for 10 min in an ice-water bath

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The transporter-mediated drug delivery approach includes 3 main strategies: (i) modification of the drug structure in a way that a specific BBB transporter will recognize it as a substrate, e.g

Y. Wanget al., Thymosin 4 released from functionalized self-assembling peptide activates epicardium and enhances repair of infarcted myocardium, Ivyspring International Publisher, 2021. doi: 10.7150/thno.52309

Over the past two decades, his continued research demonstrated that traditional high-speed centrifugation used in the L-PRF protocol led to lower leukocyte yields and diminished growth factor release over time, making the original protocol outdated compared to modern advancements [1]

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