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Monitor your progress by taking photographs at regular intervals, which provides more objective assessment than daily observation and can help determine whether hair density is genuinely declining or stabilising

Third, draw the calculated volume and add it slowly against the vial wall

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Metabolic analysis Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured with a 24-well XF Extracellular Flux Analyzer (Agilent Technologies) and Seahorse Wave Controller Software (Agilent Technologies), according to the manufacturers instructions