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These abnormal mast cells begin to degranulatereleasing massive amounts of inflammatory mediators like histamine, tryptase, and prostaglandinsin response to benign triggers, including normal dietary proteins and the physical act of digestion

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Upon reaching confluence, cultures were incubated with the adipogenesis induction cocktail (growth medium supplemented with 5 g ml 1 insulin, 1 M dexamethasone, 0.5 mM isobutylmethyxanthine and 1 M rosiglitazone) for day 12 of differentiation