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CKD is accompanied by pervasive risk factors, especially in those patients having hypertension [7], diabetes mellitus [8], and depression [9], as well as acute and chronic obstructive pulmonary disease (COPD) [10]

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Chandy estimates that 1 in 10 people experience this effect

Trypsin/LysC digestion and streptavidin enrichment Samples were digested by adding 3 l of 100 mM CaCl 2 (final concentration: 1 mM) and 2 g of trypsin/LysC (20 g trypsin/LysC resuspended in 40 l trypsin/LysC buffer, then adding 4 l to each sample), followed by shaking at 37 C for 16 hours

Kartha LB, Chandrashekar L, Rajappa M, Menon V, Thappa DM, Ananthanarayanan PH