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The (His) 6 -tag was cleaved using overnight TEV digestion, followed by ion-exchange chromatography, and finally SEC using a Superdex 200 column (HiLoad 16/600, Cytiva)

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Statistical analyses were performed with the GraphPad 5.0 software (La Jolla, CA, USA) with ANOVA non-parametrical test and then unpaired Students t -test

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