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[DOI] [PubMed] [Google Scholar] 60.Marengo B, de Ciucis C, Ricciarelli R, et al

After microscopic examination, oocytes at metaphase II stages (MII, with the polar body extrusion) were collected and cultured in 500 l of fertilization droplets as SOF medium supplemented with 5% fetal bovine serum, 2 mM sodium pyruvate, 10 g/ml heparin sodium and 1 mM caffeine in a CO 2 incubator (38.5C, 90%N 2 , 5%CO 2 and 5%O 2 ) (32)

doi: 10.1016/j.foodchem.2022.133443 386 VaithiyalingamMSumathiDLSabarathinamS

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