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The fluorescence readout of the sensors was recorded using the following excitation and emission bands: Peredox, NAPstar4.3 and NAPstarC: TS: Ex = 400 5 nm, Em = 520 5 nm

The sample was left to incubate in the dark for 25 min at ambient temperature

But we still propose that TRX may have access to the side groups of Cys190 and likely Cys168, ( Figure 7B )

Another crucial step to remember is consistency

The derived pharmacokinetic parameters, CL/F and V z /F also varied across the renal function groups with no apparent pattern

Since it can suppress gp91phox (a plasma membrane subunit of NADPH oxidase) expression and boost SOD activity, berberine is able to restore cellular redox activity (222) ( Supplementary Table 1 )