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There is, per ClinicalTrials.gov, no completed human efficacy trial for 5-Amino-1MQ

Points against their use depend on the side effects due to the deep suppression of estrogen production, like hot flashes and bone loss, often needing the use of add-back therapy for long treatments (when more than 6 months long), consisting of supplementation of hormones to reduce these negative consequences [66]

Therefore, further research is needed to assess the potential benefits of supplementation

2 The adjuvant activity of KL1 was consistent across compound sources

Reconstitution for Research Reconstitute only under an institutionally approved laboratory protocol using the protocol-defined diluent and volume

To collect the supernatant, homogenate was centrifuged at 12,000 g 5 min at 4 C