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After the membrane was blocked with 5% skim milk TBST (20 mM Tris-Cl (pH 7.4), 150 mM NaCl, 0.1% Tween-20), it was incubated with streptavidin (SA) coupled to horseradish peroxidase (HRP) (1:1000, Pierce) and washed three times with TBST for 10 min

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The cytokine storm and the oxidative stress condition induced by increased cellular ROS levels cooperate in triggering cell death [123,124]

During the hotter months in order to insure the safety and integrity of certain heat sensitive products and prevent melting, deterioration or rancidity, it may require we pack product with freeze-packs