For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min
Dispose of each syringe after its use by placing in a puncture proof container such as a coffee can or a sharps container to return to the clinic for disposal in a bio-waste container
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[15] Serious but infrequent events-such as angioedema, bronchospasm, hypotension, or urticaria-reflect IgE-mediated hypersensitivity and may require desensitization or substitution with hydroxocobalamin
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Clinical research has demonstrated that a reduction in CBF occurs during the initial phases of the disease, suggesting a connection to Syn pathology and the aberrant activation of pericytes [27, 235, 236]
