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Simply avoid that specific spot and use nearby areas instead

10.1016/j.cmet.2022.09.021 117 YangF.XiaoY.DingJ

Introducing steric blockade has proven a successful strategy to increase the stability of a peptide in vivo but typically this has been at the expense of potency, as for instance a 48-fold difference at human GLP-1R in transfected CHO cells between Ab#2_DSB#1 and the same molecule without the disulphide bridge (Ab#2_EX4)

No direct effect

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