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Dose escalation beyond the receptor saturation threshold increases systemic exposure, raises the risk of off-target effects (HGF receptors exist in liver, kidney, and vascular tissue), and wastes expensive research compounds

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Non-coding RNAs add further regulatory complexity: specific miRNA profiles (let-7 family, miR-372373 cluster) correlate with oocyte competence and embryo potential [170,171,172,173,174,175,176], while long non-coding RNAs such as NORAD and NEAT1 regulate chromatin organization [177,178,179,180]

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