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Alternative concentrations and when to use them The 3 mL standard works for most researchers
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Two different conditions were employed: endpoint A, where the medium was supplemented with an excipient (citrate-phosphate buffer, pH 5, Merck, Darmstadt, Germany), and endpoint B, where the medium was supplemented with cyanocobalamin (Merck) at a final concentration of 1 nM
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However, our bodies cannot produce B12, so we must obtain it from our diet or supplements
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