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Histologic findings were diagnostic for genital lentiginosis

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Mechanism-Based Inhibition of Human Steroid 5-Reductase by Finasteride: Enzyme-Catalyzed Formation of NADPDihydrofinasteride, a Potent Bisubstrate Analog Inhibitor.J.Amer.Chem.Soc.1996,118, 2359-2365 78

The sections were air-dried, washed three times for 5 min each in 0.3% Triton X-100 in PBS (PBS-Triton), and then blocked with 1% bovine serum albumin (BSA) in PBS for 2 h at room temperature