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There was no consistent pattern of acquisition method used across studies

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Heliyon 8 (6), e09575

And my answer is going to circle back to what you asked me before about the disease of obesity

No strong clinical trials have confirmed significant weight loss from lipotropic injections alone, according to Healthline

However, levels may be depleted by kidney and liver disease, genetic defects, valproic acid (an epilepsy drug), cancerand advancing age