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To minimize confounding factors related to the estrous cycle and ensure experimental consistency, we used only male mice in this study

L.HarrisJ

A high level of intracellular glutamate during the early stages of the disease therefore may be reflective of excessive glutamate release which could lead to excitotoxic damage

M.MaghmomehA

Next, 900 l serum extract was dried in a vacuum evaporator (Thermo), reconstituted in 90 l of 50% acetonitrile (A955, Fisher Scientific) and transferred to glass vials for LCMS analysis

In the cytosol the acetyl-CoA can be converted to malonyl-CoA via the action of acetyl-CoA carboxylase (ACC)