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B12 deficiency is remarkably prevalent in India more so than in Western populations for several reasons: Dietary Risk: Strict vegetarians and vegans: B12 is found almost exclusively in animal-derived foods (meat, fish, eggs, dairy)

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Onset: gradual

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The composite sample was then resuspended in Maxpar mass cytometry grade water supplemented with 1:10 EQ Four Element Calibration Beads (Fluidigm), filtered through a 40 m pore size cell strainer (Corning) and acquired on a Helios CyTOF system (Fluidigm)