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Park SY, Jang HL, Lee JH, Choi Y, Kim H, Hwang J, Seo D, Kim S, Nam JS
Design of reversible, cysteine-targeted Michael acceptors guided by kinetic and computational analysis
Several metal-based nanoparticles harboring multivalent elements, such as Cu 2+ /Cu + , Mn 4+/3+ /Mn 2+ , Mo 6+ /Mo 4+ , and Ir 4+ /Ir 3+ , possess strong oxidization, and can directly react with GSH (Nie et al., 2022)
The glutathionylated/oxidized enzymes were again purified via Ni-NTA affinitiy chromatograpghy followed by desalting on a PD-10 column as described above
Always disclose planned glutathione to your provider before initiating either product
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