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These identical lanes were then either stained with Coomassie or transferred onto nitrocellulose and probed with the anti-GspD antibody

The patients/participants provided their written informed consent to participate in this study

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At specific time points (0, 4, 8, 24 and 48 hours), 1.0 mL of nanoparticle suspension was centrifuged at 19354 g for 10 minutes (Centurion Scientific Ltd., UK)