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DIV12 the neurons were fixed (4% paraformaldehyde, 3% sucrose, 60 mM PIPES, 25 mM HEPES, 5 mM EGTA, 1 mM MgCl 2 , pH 7.4) for 20 min at room temperature and mounted
The samples were subsequently digested with a 1:1001:500 ratio of enzymes for 30 min at room temperature, after which the reaction was terminated for Western blot analysis
Scientists conducting research must ensure their experimental protocols comply with research-use regulations
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