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Mechanism research remains active
Grafting trials were done using wildtype, nicotine-producing and low-producing plants (a622 and pmt mutants), for which sterile seedlings growing in culture media were cut to produce scions and rootstocks that were joined in recovery plates
On the other hand, NPW treatment depressed the degree of lipid peroxidation, neutrophil infiltration and pro-inflammatory cytokine levels, and elevated antioxidant capacity in conjunction with the downregulation of gastric COX-1 and COX-2 mRNA levels, suggesting that improved gastric damage due to NPW could be eliminating the need for compensatory upregulation of COX enzymes