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They can appear as: Trace impurities from raw materials, degradation products formed when molecules break down, or microbial metabolites created when bacteria or fungi metabolize ingredients or carriers

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Such a proximal Lys1269-Glu1273 arrangement (even a transient one) would be expected to be less favorable for WD40 binding, given that the bound state of the S tail in our X-ray structures has Lys1269 and Thr/Glu1273 in a distal configuration