Biochem Biophys Res Commun 2015;466:33-9.ArticlePubMed 35
What are the side effects of IM glutathione
Biochemically it serves two enzymes: methionine synthase, which remethylates homocysteine to methionine and regenerates the cells methyl-donor pool, and methylmalonyl-CoA mutase in mitochondrial energy metabolism[1][2]
A 12.5 gram pellet of cells was resuspended in 37.5 ml buffer T (25 mM Tris-HCl, pH 7.5, 10% glycerol, 0.5 mM EDTA, 2 mM 2-Mercaptoethanol, 0.01% Igepal CA-630) containing 500 mM KCl and a cocktail of protease inhibitors (PIs) (2 g/ml each of aprotinin, chymostatin, leupeptin, and pepstatin A) and lysed with a Dounce homogenizer and brief sonication
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