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Upon ligand binding, the nanoparticle-receptor complex is internalized via clathrin-coated vesicles to be transported across the BBB and subsequently released by exocytosis into the brain parenchyma 119,120

This is a localised and transient chemical response, not an allergic reaction and not a sign of peptide instability

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The activity of phase II enzymes, like UGTs, may also change during pregnancy

The secondary antibodies used are as follows: Goat Anti-Mouse IgG H&L (Alexa Fluor 488) (Abcam, ab150113), Goat Anti-Mouse IgG H&L (Alexa Fluor 647) (Abcam, ab150115), Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (Abcam, ab150077), Donkey Anti-Rabbit IgG H&L (Alexa Fluor 647) (Abcam, ab150075)