A volume of 350 L of 0.1 M potassium phosphate buffer (pH 7.4), 50 L of 1 mM substrate (diclofenac for CYP2C9 assay and dextromethorphan for CYP2D6, both substrates purchased from Sigma-Aldrich, USA) and 50 L of 2.5 mg/mL microsome (obtained from rat livers from respective groups) were mixed separately in Eppendorf tubes
Besides having the high-cost issue, the antibody might also interact with the endogenous proteins and cause non-specificity
Li, D., Mller, M
Dechent P, Pouwels PJ, Wilken B, Hanefeld F, Frahm J: Increase of total creatine in human brain after oral supplementation of creatine-monohydrate
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If you drink heavily or have alcohol use disorder, your provider may recommend abstaining for several weeks before the procedure to allow your body to stabilize