Subcutaneous protocols sometimes reference 100 to 500 mcg per administration, but there is no validated subcutaneous dosing established by controlled trials
DPCs secrete signaling proteins, such as the wingless-INT (Wnt) family, that promote the proliferation and differentiation of surrounding stromal cells and induce HFs to enter new growth phases (Kim et al., 2016)
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the lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C
