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At day 6, medium was changed into neuronal maintenance medium consisting of: Neurobasal (Gibco TM , Thermo Fisher Scientific, Netherlands #21103049) containing 1:50 B-27 supplement without vitamin A (Gibco TM , Thermo Fisher Scientific, Netherlands #12587010) and 1:100 GlutaMax (Gibco TM , Thermo Fisher Scientific, Netherlands #35050061)